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“百万微生态”国际合作计划（MMHP）

肠道菌群是一个庞大复杂的生态系统，它占全身菌群总数80%左右，被称为人体的“第二基因组”。从2006年第一个Human gut Microbiome项目的启动，到2010年欧盟MetaHIT计划（人体肠道宏基因组计划），到2019年“百万微生态”国际合作计划（MMHP），展现了全球对肠道菌群关注度的提升。

“百万微生态”国际合作计划（MMHP）是目前全球规模最大的国际微生态研究计划，旨在通过华大智造独家提供的DNBSEQ™微生物宏基因组测序技术，绘制不同种族、年龄、性别以及健康状态下的人体微生物图谱，建立大规模人群水平的微生态研究基线，从而推动人体微生态领域的前沿转化医学研究。致力于在3-5年内对100万份来自肠道、口腔、皮肤、生殖道等人体器官的微生物组样品进行测序分析，由21个机构和10多个参与国家共同努力，目前基于华大智造测序平台已完成样本量测序超13万例。

*数据统计截至2023年12月

项目经验（持续更新）

DNESEQ^TM技术优势

MMHP样本的性能分析结果证实了华大智造DNBSEQ™测序平台具有高度一致性的表现。与常规DNA阵列相比，DNBSEQ™测序技术具备低重复序列、低标签跳跃、信号密度高、平衡高通量和高质量数据的技术优势，基于此，参与机构能够扩展宏基因组学的应用和能力，有助于进一步开展相关研究和精准医疗。

产品组合

在支持MMHP项目推进的过程中，针对粪便样本的宏基因组测序工作全流程，华大智造提供了一整套经过验证的、一站式工具箱，助力微生物宏基因组研究。2024年，华大智造重磅推出人类微生物宏基因组测序组合产品，基于独有的DNBSEQ™测序技术，涵盖样本采集、提取、建库、测序和分析全流程，涵盖中、高、超高通量测序需求，是大规模人体微生态研究的理想工具。

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重要科研成果展示

Nature communications | A genome and gene catalog of the aquatic microbiomes of the Tibetan Plateau
In April 2024, Professor Zheng Hao from Kunming University of Science and Technology, in collaboration with MGI and other institutions, published an article titled "Identification of the mutual gliding locus as a factor for gut colonization in non-native bee hosts using ARTP mutagenesis" in the journal Microbiome. The study employed atmospheric and room temperature plasma (ARTP) mutagenesis, metagenomic sequencing, amplicon sequencing, and colony expansion assays. It revealed that mutations in the mglB gene, encoding the mutual gliding motility protein B, provide Snodgrassella strains with a competitive advantage in gut colonization of non-native hosts, suggesting a potential role of T4P-dependent cell motility in establishing microbiota-host association. This research elucidates the genetic mechanisms of gut colonization by Snodgrassella strains in non-native hosts and offers an experimental framework for future studies on host-microbe interactions. The project utilized the DNBSEQ-E25 sequencing platform from MGI for amplicon sequencing of the mutation region in the mglB gene, further validating the results of microbial competition assays.
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Nature Communications | A robust yeast biocontainment system with two-layered regulation switch dependent on unnatural amino acid
Synthetic auxotrophy in which cell viability depends on the presence of an unnatural amino acid (unAA) provides a powerful strategy to restrict unwanted propagation of genetically modified organisms (GMOs) in open environments and potentially prevent industrial espionage. On October 14th, 2023, the University of Chinese Academy of Sciences, BGI Research, and the Guangdong Provincial Key Laboratory, among others, jointly published an article titled "A Robust Yeast Biocontainment System with Two-Layered Regulation Switch Dependent on Unnatural Amino Acid" in Nature Communications. This publication underscores that the utilization of their multiplex safeguard system could restrict the proliferation of strains of interest in a real fermentation scenario, highlighting the great potential of yeast biocontainment strategy to protect the industrial proprietary strains.
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LWT | Evaluation of the safety and probiotic properties of Lactobacillus gasseri LGZ1029 based on whole genome analysis
South China University of Technology published an article titled "Evaluation of the safety and probiotic properties of Lactobacillus gasseri LGZ1029 based on whole genome analysis" in LWT-FOOD SCIENCE AND TECHNOLOGY. The team reported the complete genome sequence of L. gasseri LGZ1029, which comprised 2,027,325 base pairs encoding 2005 genes, with an average G + C content of 35.06%. Their analysis of genes related to antibiotic resistance, virulence factors, and harmful metabolite production led them to speculate on the safety of L. gasseri LGZ1029. Furthermore, genome mining revealed the presence of a complete plnABCD operon, suggesting its potential to produce antimicrobial bacteriocins. Stress-related genes indicated favorable gastrointestinal tolerance, bacteriostatic abilities, and antioxidant activity, all indicative of excellent probiotic characteristics, positioning L. gasseri LGZ1029 as a promising probiotic candidate.
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